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hesc line sa002  (Cellartis)

 
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    Cellartis hesc line sa002
    Hesc Line Sa002, supplied by Cellartis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hesc+line+sa002/human+embryonic+stem+cell+line+sa002/pm24482287-38-20-23
    Average 90 stars, based on 1 article reviews
    hesc line sa002 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: A simple method for large-scale generation of dopamine neurons from human embryonic stem cells.
    Article Snippet: Dopamine (DA) neurons derived from human embryonic stem cells (hESCs) are potentially valuable in drug screening and as a possible source of donor tissue for transplantation in Parkinson’s disease.. However, existing culture protocols that promote the differentiation of DA neurons from hESCs are complex, involving multiple steps and having unreliable results between cultures.. Here we report a simple and highly reproducible culture protocol that induces expandable DA neuron progenitors from hESCs in attached cultures.

    Cell Culture:

    Article Title: Assaying cardiac biomarkers for toxicity testing using biosensing and cardiomyocytes derived from human embryonic stem cells.
    Article Snippet: Human embryonic stem cell (hESC) derived cardiomyocytes are in the present study being used for testing drug-induced cardiotoxicity in a biosensor set-up.. The design of an in vitro testing alternative provides a novel opportunity to surpass previous methods based on rodent cells or cell lines due to its significantly higher toxicological relevance.. In this report we demonstrate how hESC-derived cardiomyocytes

    Irradiation:

    Article Title: Adipocytes derived from PA6 cells reliably promote the differentiation of dopaminergic neurons from human embryonic stem cells.
    Article Snippet: The PA6 stromal cell line comprises a heterogeneous population of cells that can induce both mouse and human embryonic stem cells to differentiate into dopaminergic neurons.. This ability of PA6 cells has been termed stromal cell-derived inducing activity (SDIA).. The level of SDIA has been found to vary considerably between and within batches of PA6 cells.



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    hesc line sa002  (Cellartis)
    90
    Cellartis hesc line sa002
    Hesc Line Sa002, supplied by Cellartis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hesc+line+sa002/human+embryonic+stem+cell+line+sa002/pm24482287-38-20-23
    Average 90 stars, based on 1 article reviews
    hesc line sa002 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    hesc lines sa002  (Cellartis)
    90
    Cellartis hesc lines sa002
    The agarose 3-D matrix support EB formation efficiency and EB growth. a EB formation efficiency of the <t>hESC</t> <t>lines</t> <t>SA002,</t> <t>AS034,</t> <t>AS038</t> and <t>SA121</t> when cultured for 1 week in suspension (S), hanging droplet (H.D) or agarose (A), respectively. Each bar represents the mean of two individual replicates where formation efficiency was calculated by dividing the number of EBs formed after 1 week by the number of colonies originally placed into the culture system. Each replicate was performed with 5–15 colonies, per hESC line, placed in each culture system. The Chi Squared test for trend was statistically significant for each hESC line (χ2 = 4,228; p < 0.050 for hESC line SA002, χ2 = 6,936; p < 0.010 for hESC line AS034, χ2 = 6,646; p < 0.010 for hESC line AS038 and χ2 = 15,026; p < 0.001 for hESC line SA121) indicating that EB formation efficiency increased when a more supportive culture milieu was used, i.e. EBs are hindered to adhere to the culture plate, agglomerate and dissolve. b EB size of the hESC lines SA002, AS034, AS038 and SA121 when cultured for 1 week in suspension (S), hanging droplet (H.D) or agarose (A), respectively. Each bar represents the mean of two individual replicates with 3 representative EBs, n = 6. *p < 0.05 with a two sample student’s t test. c The agarose 3-D matrix support differentiation of EBs from hESC. Histological sections stained with hematoxylin-eosin were made of EBs from the 4 hESC lines; SA002, AS034, AS038 and SA121 grown in agarose or hanging droplets for 1 week. The EBs cultured in agarose showed higher developed structures and higher extracellular matrix production (arrows) than EBs cultured in the hanging droplet system. Bar 100 μm
    Hesc Lines Sa002, supplied by Cellartis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hesc+line+sa002/human+embryonic+stem+cell+line+sa002/pmc03081049-117-2-17
    Average 90 stars, based on 1 article reviews
    hesc lines sa002 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    hescs cell line sa002  (Cellartis)
    90
    Cellartis hescs cell line sa002
    The agarose 3-D matrix support EB formation efficiency and EB growth. a EB formation efficiency of the <t>hESC</t> <t>lines</t> <t>SA002,</t> <t>AS034,</t> <t>AS038</t> and <t>SA121</t> when cultured for 1 week in suspension (S), hanging droplet (H.D) or agarose (A), respectively. Each bar represents the mean of two individual replicates where formation efficiency was calculated by dividing the number of EBs formed after 1 week by the number of colonies originally placed into the culture system. Each replicate was performed with 5–15 colonies, per hESC line, placed in each culture system. The Chi Squared test for trend was statistically significant for each hESC line (χ2 = 4,228; p < 0.050 for hESC line SA002, χ2 = 6,936; p < 0.010 for hESC line AS034, χ2 = 6,646; p < 0.010 for hESC line AS038 and χ2 = 15,026; p < 0.001 for hESC line SA121) indicating that EB formation efficiency increased when a more supportive culture milieu was used, i.e. EBs are hindered to adhere to the culture plate, agglomerate and dissolve. b EB size of the hESC lines SA002, AS034, AS038 and SA121 when cultured for 1 week in suspension (S), hanging droplet (H.D) or agarose (A), respectively. Each bar represents the mean of two individual replicates with 3 representative EBs, n = 6. *p < 0.05 with a two sample student’s t test. c The agarose 3-D matrix support differentiation of EBs from hESC. Histological sections stained with hematoxylin-eosin were made of EBs from the 4 hESC lines; SA002, AS034, AS038 and SA121 grown in agarose or hanging droplets for 1 week. The EBs cultured in agarose showed higher developed structures and higher extracellular matrix production (arrows) than EBs cultured in the hanging droplet system. Bar 100 μm
    Hescs Cell Line Sa002, supplied by Cellartis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hesc+line+sa002/human+embryonic+stem+cell+line+sa002/10__1089_slash_ten__tec__2010__0437-49-4-5
    Average 90 stars, based on 1 article reviews
    hescs cell line sa002 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

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    The agarose 3-D matrix support EB formation efficiency and EB growth. a EB formation efficiency of the hESC lines SA002, AS034, AS038 and SA121 when cultured for 1 week in suspension (S), hanging droplet (H.D) or agarose (A), respectively. Each bar represents the mean of two individual replicates where formation efficiency was calculated by dividing the number of EBs formed after 1 week by the number of colonies originally placed into the culture system. Each replicate was performed with 5–15 colonies, per hESC line, placed in each culture system. The Chi Squared test for trend was statistically significant for each hESC line (χ2 = 4,228; p < 0.050 for hESC line SA002, χ2 = 6,936; p < 0.010 for hESC line AS034, χ2 = 6,646; p < 0.010 for hESC line AS038 and χ2 = 15,026; p < 0.001 for hESC line SA121) indicating that EB formation efficiency increased when a more supportive culture milieu was used, i.e. EBs are hindered to adhere to the culture plate, agglomerate and dissolve. b EB size of the hESC lines SA002, AS034, AS038 and SA121 when cultured for 1 week in suspension (S), hanging droplet (H.D) or agarose (A), respectively. Each bar represents the mean of two individual replicates with 3 representative EBs, n = 6. *p < 0.05 with a two sample student’s t test. c The agarose 3-D matrix support differentiation of EBs from hESC. Histological sections stained with hematoxylin-eosin were made of EBs from the 4 hESC lines; SA002, AS034, AS038 and SA121 grown in agarose or hanging droplets for 1 week. The EBs cultured in agarose showed higher developed structures and higher extracellular matrix production (arrows) than EBs cultured in the hanging droplet system. Bar 100 μm

    Journal: Cytotechnology

    Article Title: Sustained embryoid body formation and culture in a non-laborious three dimensional culture system for human embryonic stem cells

    doi: 10.1007/s10616-011-9344-y

    Figure Lengend Snippet: The agarose 3-D matrix support EB formation efficiency and EB growth. a EB formation efficiency of the hESC lines SA002, AS034, AS038 and SA121 when cultured for 1 week in suspension (S), hanging droplet (H.D) or agarose (A), respectively. Each bar represents the mean of two individual replicates where formation efficiency was calculated by dividing the number of EBs formed after 1 week by the number of colonies originally placed into the culture system. Each replicate was performed with 5–15 colonies, per hESC line, placed in each culture system. The Chi Squared test for trend was statistically significant for each hESC line (χ2 = 4,228; p < 0.050 for hESC line SA002, χ2 = 6,936; p < 0.010 for hESC line AS034, χ2 = 6,646; p < 0.010 for hESC line AS038 and χ2 = 15,026; p < 0.001 for hESC line SA121) indicating that EB formation efficiency increased when a more supportive culture milieu was used, i.e. EBs are hindered to adhere to the culture plate, agglomerate and dissolve. b EB size of the hESC lines SA002, AS034, AS038 and SA121 when cultured for 1 week in suspension (S), hanging droplet (H.D) or agarose (A), respectively. Each bar represents the mean of two individual replicates with 3 representative EBs, n = 6. *p < 0.05 with a two sample student’s t test. c The agarose 3-D matrix support differentiation of EBs from hESC. Histological sections stained with hematoxylin-eosin were made of EBs from the 4 hESC lines; SA002, AS034, AS038 and SA121 grown in agarose or hanging droplets for 1 week. The EBs cultured in agarose showed higher developed structures and higher extracellular matrix production (arrows) than EBs cultured in the hanging droplet system. Bar 100 μm

    Article Snippet: The 5 hESC lines (SA002, AS034, AS038, SA121, and SA167) used in this study were established at Cellartis AB, Gothenburg, Sweden (Heins et al. 2004 ; Sjogren-Jansson et al. 2005 ).

    Techniques: Cell Culture, Suspension, Staining

    Overview of the agarose culture. a Schematic procedure according to materials and methods. The numbers in boxes indicates the procedure order. b Low magnification image of a 2 days old agarose culture of EBs from the SA002 hESC line. Each EB is fixed in place and can be followed individually through out the culture time and harvested individually at any time point. Bar 1,000 μm

    Journal: Cytotechnology

    Article Title: Sustained embryoid body formation and culture in a non-laborious three dimensional culture system for human embryonic stem cells

    doi: 10.1007/s10616-011-9344-y

    Figure Lengend Snippet: Overview of the agarose culture. a Schematic procedure according to materials and methods. The numbers in boxes indicates the procedure order. b Low magnification image of a 2 days old agarose culture of EBs from the SA002 hESC line. Each EB is fixed in place and can be followed individually through out the culture time and harvested individually at any time point. Bar 1,000 μm

    Article Snippet: The 5 hESC lines (SA002, AS034, AS038, SA121, and SA167) used in this study were established at Cellartis AB, Gothenburg, Sweden (Heins et al. 2004 ; Sjogren-Jansson et al. 2005 ).

    Techniques:

    Diagram of the EB growth of the 4 hESC lines when cultured in agarose 3-D matrix for 8 weeks. a Growth of the hESC lines SA002, AS034, SA167, SA121 when cultured in agarose for 8 weeks. All measure points represent the mean of six representative EBs. (filled circle) symbols the average size of the 4 hESC lines after 1 week of suspension culture from Fig. 2. (Open square) symbols the average size of the 4 hESC lines after 1 week of hanging droplet culture from Fig. 2. b The EBs from the 4 hESC lines; SA002, AS034, SA121 and SA167 showed all intact nuclei (black arrows) in the middle of the EBs cultured in agarose 3-D matrix for 8 weeks, here represented by the AS0034 hESC line. Staining = Hematoxylin-Eosin. Bar 10 μm

    Journal: Cytotechnology

    Article Title: Sustained embryoid body formation and culture in a non-laborious three dimensional culture system for human embryonic stem cells

    doi: 10.1007/s10616-011-9344-y

    Figure Lengend Snippet: Diagram of the EB growth of the 4 hESC lines when cultured in agarose 3-D matrix for 8 weeks. a Growth of the hESC lines SA002, AS034, SA167, SA121 when cultured in agarose for 8 weeks. All measure points represent the mean of six representative EBs. (filled circle) symbols the average size of the 4 hESC lines after 1 week of suspension culture from Fig. 2. (Open square) symbols the average size of the 4 hESC lines after 1 week of hanging droplet culture from Fig. 2. b The EBs from the 4 hESC lines; SA002, AS034, SA121 and SA167 showed all intact nuclei (black arrows) in the middle of the EBs cultured in agarose 3-D matrix for 8 weeks, here represented by the AS0034 hESC line. Staining = Hematoxylin-Eosin. Bar 10 μm

    Article Snippet: The 5 hESC lines (SA002, AS034, AS038, SA121, and SA167) used in this study were established at Cellartis AB, Gothenburg, Sweden (Heins et al. 2004 ; Sjogren-Jansson et al. 2005 ).

    Techniques: Cell Culture, Suspension, Staining

    EBs from the 4 hESC lines; SA002, AS034, SA121 and SA167 cultured in agarose for 8 weeks developed heterogeneous differentiation patterns. Histological analysis with Alcian Blue-van Gieson staining showed differentiation heterogeneity both between EBs from the 4 hESC lines and between different EBs from the same hESC line. EBs from the AS034, SA121 and SA167 in three individual replicates developed highly differentiated areas with cell types from the three germ layers (thin black arrow for neuronal rosettes like structures representing ectoderm, white arrow for connective tissue representing mesoderm and thick black arrow for epithelia representing endoderm). The EBs from the SA002 cell line did not develop highly differentiated structures but produced extracellular matrix. Bar 100 μm

    Journal: Cytotechnology

    Article Title: Sustained embryoid body formation and culture in a non-laborious three dimensional culture system for human embryonic stem cells

    doi: 10.1007/s10616-011-9344-y

    Figure Lengend Snippet: EBs from the 4 hESC lines; SA002, AS034, SA121 and SA167 cultured in agarose for 8 weeks developed heterogeneous differentiation patterns. Histological analysis with Alcian Blue-van Gieson staining showed differentiation heterogeneity both between EBs from the 4 hESC lines and between different EBs from the same hESC line. EBs from the AS034, SA121 and SA167 in three individual replicates developed highly differentiated areas with cell types from the three germ layers (thin black arrow for neuronal rosettes like structures representing ectoderm, white arrow for connective tissue representing mesoderm and thick black arrow for epithelia representing endoderm). The EBs from the SA002 cell line did not develop highly differentiated structures but produced extracellular matrix. Bar 100 μm

    Article Snippet: The 5 hESC lines (SA002, AS034, AS038, SA121, and SA167) used in this study were established at Cellartis AB, Gothenburg, Sweden (Heins et al. 2004 ; Sjogren-Jansson et al. 2005 ).

    Techniques: Cell Culture, Staining, Produced

    Agarose culture of EBs support formation of the three germ layers. Representative immunohistochemistry staining of a AFP (white arrow), b vimentin and c β-tubulin in EBs from the SA121 hESC line. Nuclear staining with DAPI. d Summary of AFP, vimentin and β-tubulin expression after 8 weeks of agarose EB culture

    Journal: Cytotechnology

    Article Title: Sustained embryoid body formation and culture in a non-laborious three dimensional culture system for human embryonic stem cells

    doi: 10.1007/s10616-011-9344-y

    Figure Lengend Snippet: Agarose culture of EBs support formation of the three germ layers. Representative immunohistochemistry staining of a AFP (white arrow), b vimentin and c β-tubulin in EBs from the SA121 hESC line. Nuclear staining with DAPI. d Summary of AFP, vimentin and β-tubulin expression after 8 weeks of agarose EB culture

    Article Snippet: The 5 hESC lines (SA002, AS034, AS038, SA121, and SA167) used in this study were established at Cellartis AB, Gothenburg, Sweden (Heins et al. 2004 ; Sjogren-Jansson et al. 2005 ).

    Techniques: Immunohistochemistry, Staining, Expressing